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Pathway Discovery Resource

July 10, 2018 by bruce Haire

Pathway Discovery Resource

Created OnJuly 10, 2018
bybruce Haire
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The Pathway Discovery Resource (PDR) is a high throughput screening facility providing cost effective screening methodologies for cell based assays.  Currently, the PDR offers cell based screening with various genetic and chemical tools.  The following major equipment is located in the PDR: one Celigo S Cell cytometer, one Perkin Elmer Zephyr compact liquid handling workstation, ThermoFisher MultiDrop, one Envision High Throughput Plate Reader equipped with optics for Fluorescence, Luminescence, Fluorescence Polarization (FP), Time Resolved Fluorescence (TRF), Absorbance, Alpha Screen, and FRET based detection in 96 or 384 well formats, one Janus dual-arm robotic liquid handling system, and one Flexdrop plate filler.

Welcome to the URMC Pathway Discovery Resource

The Pathway Discovery Resource (PDR) is a high throughput screening facility providing cost effective screening methodologies for cell based assays.  Currently, the PDR offers cell based screening with various genetic and chemical tools.  The following major equipment is located in the PDR: one Celigo S Cell cytometer, one Perkin Elmer Zephyr compact liquid handling workstation, ThermoFisher MultiDrop, one Envision High Throughput Plate Reader equipped with optics for Fluorescence, Luminescence, Fluorescence Polarization (FP), Time Resolved Fluorescence (TRF), Absorbance, Alpha Screen, and FRET based detection in 96 or 384 well formats, one Janus dual-arm robotic liquid handling system, and one Flexdrop plate filler.  The PDR is Co-directed by two Faculty-level Instructors experienced in high throughput cell and molecular biology techniques.  In addition, the PDR is operated by a full time Technical Associate with significant experience in high throughput technologies.  The PDR integrates with various other shared resource facilities at University of Rochester including; genomics, flow cytometry, and proteomics.

In addition, GRC staff lead collaborative projects with URMC investigators to develop new methodologies and incorporate emerging genomic technologies into faculty research programs and the GRC workflow. Dedicated computational support for hardware, data analysis and storage of high-throughput sequence data is provided by the Center for Integrated Research Computing (CIRC).

The UR Genomics Research Center (GRC) provides collaborative assistance with experimental design and data analysis for investigators using high-throughput next generation sequencing (NGS), genotyping and gene expression in their research programs.

About the Multiphoton SRL

The mission of the URMC Multiphoton Shared Resource Laboratory (SRL) is to provide state-of-the-art multiphoton imaging capabilities to further the biomedical and bio-optical research at the URMC and the University of Rochester with emphasis on intravital imaging and systems physiology.

The Multiphoton SRL provides access to an Olympus Fluoview 1000 AOM-MPM imaging system and a Spectra-Physics MaiTai HP DeepSee Ti:Sa laser system with dispersion compensation. Further capabilities include engineering applications for opto-electronics and spectroscopy, and software development for quantitative image analysis, visualization and instrument control.

Training and access to the imaging system are available as well as support in the design of experimental procedures, acquisition of image data, and analysis of the required images. Our services are available to all investigators within the University as well as academic or commercial/industrial institutions in the region.

The use of laboratory animals covered by active UCAR-protocols, tissue explants, and cell-cultures, and radio-active tracer substances is permitted. We also offer access to a chemical hood. Please ensure compliance with all intramural and extramural regulations regarding use of research animals and hazardous materials before accessing the Multiphoton SRL. Contributions made by the core facility should be briefly mentioned in the acknowledgments of scientific publications. If the work performed in the core facility is more than routine, publication as co-author is appropriate.

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