• Skip to primary navigation
  • Skip to main content
  • Skip to footer
Rock and Roll City Mattress

Rock and Roll City Mattress

Rock and Roll City Mattress Cleveland's #1 rated mattress store

  • Home
  • Confused? Click Here
  • About Us
    • About Us
    • Media Advertising
    • Our Service
    • Testimonials
    • Wake Up Happy
    • Warranties
  • Store
    • Factory Wholesale-Closeouts
      • Brooklyn Bedding
      • Corsicana
      • Englander
      • Malouf
      • Symbol
    • Famous Brands-Sale Section
      • AWARA
      • Dreamcloud
      • Ghost Bed
      • Nectar
      • Serta
    • Sleep Systems-Packages
      • BEDTECH
      • Blu Sleep
      • Mlily
      • Personal Comfort
      • Kingsdown
    • Adjustable Base
    • Mattresses
    • Sleep Accessories
      • Pillows
    • Furniture
  • Finance Payments
  • New Account
  • Contact Us
    • 440-725-7290
  • $0.00

Biomolecular Interaction facility

July 10, 2018 by bruce Haire

Biomolecular Interaction facility

Created OnJuly 10, 2018
bybruce Haire
You are here:
  • KB Home
  • Research Labs
  • Resource Labs & Facilities
  • Biomolecular Interaction facility
< Back

Our primary service is to provide access to our large, human single-chain Fv antibody library and to perform custom enrichments on user-provided target antigens. We typically generate 3- 10 distinct scFvs against protein targets that can be expressed in E. coli for a variety of applications. We have the capability of also expressing selected scFvs as scFv-Fc in mammalian cells. In addition to phage antibody technology, we can also perform enrichments using peptide libraries.

Mission Statement

The Biomolecular Interaction core facility seeks to give access to custom affinity reagents to researchers in the University. We utilize phage display to generate recombinant antibodies to user provided target molecules and produce the single-chain Fv proteins for the user. We also can perform affinity measurements using surface plasmon resonance or provide access to trained users to this equipment.

Core Services

Our primary service is to provide access to our large, human single-chain Fv antibody library and to perform custom enrichments on user-provided target antigens. We typically generate 3- 10 distinct scFvs against protein targets that can be expressed in E. coli for a variety of applications. We have the capability of also expressing selected scFvs as scFv-Fc in mammalian cells. In addition to phage antibody technology, we can also perform enrichments using peptide libraries.

A secondary service is to determine the affinity of protein-protein interactions using surface plasmon resonance analysis. We have a Reichert SR7000DC refractometer that is utilized either by core staff, or by special arrangement can be used by trained users for a daily rental fee.

Specialized Equipment

  • Reichert SR7000 Dual Channel surface plasmon resonance refractometer
  • Bio-Rad DuoFlow chromatography system
  • BioRobotics automated colony picker
  • PanVera fluorescence polarization spectrophotometer

Faculty User Group

Not formally established. Major users of the core have been Alan Smrcka, Ben Miller, Fred Hagen, and Michael Pichichero. Two industrial contracts are on-going.

The Multiphoton Core Facility is currently undergoing a major upgrade to house an Olympus Fluoview FVMPE-RS Twin Lasers Gantry System which will be equipped with the current MaiTai HP DeepSee Ti:Sa laser and a second Insight X3 laser. The new system will have galvo and resonant scanners for both structure and dynamics imaging. The FVMPE-RS system combines high speed, deep observation capability with multi-color imaging, which will meet a myriad of experiment needs. System installation is planned to be done in early December, and full operation is expected in January 2018. You are welcome to schedule a meet with the director if you are interested in using our upcoming new two-photon microscope.

Footer

Rock & Roll City Mattress Company

Come to visit us for a great experience finding your next mattress, whether it’s for the kids, the spare room or the master bedroom, we have it & you will be pleasantly surprised at the low prices & friendly service.

Follow us

  • facebook
  • instagram

Useful Links

  • Dreamcloud
  • Nectar Mattress
  • Personal Comfort
  • Brooklyn Bedding
  • Contact Us

Rockin Newsletter

Loading

Rock And Roll City Mattress 2023. All Rights Reserved. - Powered by WM Advertising