Biomolecular Interaction facility
Our primary service is to provide access to our large, human single-chain Fv antibody library and to perform custom enrichments on user-provided target antigens. We typically generate 3- 10 distinct scFvs against protein targets that can be expressed in E. coli for a variety of applications. We have the capability of also expressing selected scFvs as scFv-Fc in mammalian cells. In addition to phage antibody technology, we can also perform enrichments using peptide libraries.
Mission Statement
The Biomolecular Interaction core facility seeks to give access to custom affinity reagents to researchers in the University. We utilize phage display to generate recombinant antibodies to user provided target molecules and produce the single-chain Fv proteins for the user. We also can perform affinity measurements using surface plasmon resonance or provide access to trained users to this equipment.
Core Services
Our primary service is to provide access to our large, human single-chain Fv antibody library and to perform custom enrichments on user-provided target antigens. We typically generate 3- 10 distinct scFvs against protein targets that can be expressed in E. coli for a variety of applications. We have the capability of also expressing selected scFvs as scFv-Fc in mammalian cells. In addition to phage antibody technology, we can also perform enrichments using peptide libraries.
A secondary service is to determine the affinity of protein-protein interactions using surface plasmon resonance analysis. We have a Reichert SR7000DC refractometer that is utilized either by core staff, or by special arrangement can be used by trained users for a daily rental fee.
Specialized Equipment
- Reichert SR7000 Dual Channel surface plasmon resonance refractometer
- Bio-Rad DuoFlow chromatography system
- BioRobotics automated colony picker
- PanVera fluorescence polarization spectrophotometer
Faculty User Group
Not formally established. Major users of the core have been Alan Smrcka, Ben Miller, Fred Hagen, and Michael Pichichero. Two industrial contracts are on-going.
The Multiphoton Core Facility is currently undergoing a major upgrade to house an Olympus Fluoview FVMPE-RS Twin Lasers Gantry System which will be equipped with the current MaiTai HP DeepSee Ti:Sa laser and a second Insight X3 laser. The new system will have galvo and resonant scanners for both structure and dynamics imaging. The FVMPE-RS system combines high speed, deep observation capability with multi-color imaging, which will meet a myriad of experiment needs. System installation is planned to be done in early December, and full operation is expected in January 2018. You are welcome to schedule a meet with the director if you are interested in using our upcoming new two-photon microscope.
